Fluoroquinolones（FQs） are usually used to prevent and treat animal diseases in livestock and aquaculture. However， excessive or irregular use of FQs can cause the production of drug-resistant strains. Moreover， the introduction of animal foodstuff with FQs residues into food chain can lead to bioaccumulative toxicity. Therefore， to explore the effective FQs detection methods has gradually become the focus of many researchers. At present， various detection methods have been established at home and abroad. The aptamer-based methods for FQs detection compared with other methods， especially， have the advantages of high sensitivity， good specificity， low cost and rapid operation. Therefore， the research progress of the worldwide aptamer-based biosensors used in FQs detection in the late five years was summarized in this review. The principle， technical application， advantages and disadvantages of diverse FQs aptamer biosensors were described in detail， covering fluorescent， colorimetric and electrochemical and other sensors. Finally， the problems existing in detection methods were discussed， and an outlook for development direction of aptamer-based FQs detection biosensors in the future was made in the review.