Glycoprotein has many kinds of biological activities such as anti-oxidation and is widely existed in organisms. At present， natural glycoproteins from various sources have been extracted and used， but the germinated brown rice glycoprotein（GBRG） has not received widespread attention because of its unknown function. Different glycoprotein peaks were further purified by DEAE-Cellucose 52 and Sepadex G-200， and the anti-inflammatory and hypoglycemic properties of each peak were studied. The results showed that three glycoprotein peaks， named WEG， SEG-1 and SEG-2， were obtained by separation and purification. The three GBRG had no toxic side effects at a concentration of 0.025-0.2 mg/mL， and all them could inhibit the relative mRNA expression levels of the inflammatory factors COX-2， iNOS， TNF-α and IL-1β mucin in mouse macrophages RAW264.7 induced by lipopolysaccharide（LPS）， indicating that GBRG had a good inhibitory effect on inflammation. The three kinds of GBRG could inhibit the activity of α-glucosidase and α-amylase better， among which SEG-2 showed the best inhibitory effect， with the inhibition rates of 65.71% and 64.5%， respectively， and the inhibition rates of half were 0.46 mg/mL and 0.912 mg/mL， respectively， but they were weaker than acarbose.