Cyclocarya paliurus is a medicine food homology plant. This study was designed to investigate the hypoglycemic and anti-inflammatory effects of Cyclocarya paliurus extract. The experimental diabetic mice were induced by streptozotocin/nicotinamide（STZ/NA）. According the results of MTT assay， the 50.0 μg/mL Cyclocarya paliurus extract （CPE） had the best proliferation rate 71.0% on RIN-m5F cells after treated for 24 h. The serological analysis demonstrated the levels of blood glucose， total cholesterol， triglycerides（TG） were significantly decreased after continuous oral of each dose of CPE for 4 weeks in the mice model of type 2 diabetes. Especially， the 200.0 mg/mL CPE group showed the best effect and significant statistical differences（P＜0.05） compared with the STZ group. In addition， compared with the 12-O-tetradecanoylphorbol-13-acetate（TPA） group， the reduction of ear edema was 26.9% and 27.2% respectively after administration of 25.0 mg/mL CPE for 12 and 24 hours in the anti-inflammatory experiment of mice. The inhibition of high dose group was better than the positive control （indomethacin）. In conclusion， a certain dose of CPE will help to inhibit inflammation， promote pancreatic cell proliferation， and protect pancreatic tissue， consequently， decrease the level of blood glucose and triglycerides， prevent the developing of diabetes and related complications. The study is helpful for the deep processing and functional food development of Cyclocarya paliurus.