Objective： To investigate the bacterial powder preparative process of inositol-producing Lactobacillus plantarum ZJ2868. Methods： Using the cell survival rate as the target， the centrifugation speed， protective agent formula， rehydration condition and storage stability for preparing the bacterial powder of L. plantarum ZJ2868 were explored. Results： The optimal centrifugation condition was 4 000 r/min for 10 min， the protective agent formula optimized was L-sodium glutamate 11%， skimmed milk 14% and trehalose 9.0%， and the optimal rehydration condition and storage temperature were rehydration for 30 min in the original protective agent and 4 ℃， respectively. The viable count， survival rate and water content of the lyophilized L. plantarum ZJ2868 bacterial powder were 2.90×109 CFU/mL， 85.4% and 3.7%， respectively. Conclusion： This study provides the experimental basis for the preparation of the L. plantarum bacterial powder.