基于多酚类物质差异性判别普洱生茶的贮藏年限
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国家自然科学基金项目(31760490)


Discrimination of Storage Life of Raw Pu-erh Tea Based on the Difference of Polyphenol
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    摘要:

    以茶叶中多酚类物质作为判别普洱茶贮藏年限的指标,开展贮藏年限研究。以18种代表性多酚类成分作为特征物质,通过高效液相色谱仪测定,结合主成分分析、聚类分析和偏最小二乘判别法研究不同贮藏年限普洱生茶中多酚类物质的变化特征。结果表明:随着贮藏时间的延长,普洱生茶中儿茶素类组分含量呈逐渐下降的趋势,茶黄素类组分含量呈逐渐上升的趋势,黄酮及黄酮苷类成分中部分组分呈下降趋势,而部分组分呈上升趋势。化学计量学分析表明:相同贮藏年限的普洱生茶样品可聚集在一起,而不同贮藏年限样品之间呈现分离结果。经偏最小二乘判别法分析,不同贮藏年限普洱生茶样品呈现明显的分离趋势。依据偏最小二乘法分析中变量(变量值>1)重要性分析结果,结合含量差异性分析结果,没食子酸、山奈酚、茶黄素-3-没食子酸酯、表没食子儿茶素、表儿茶素、没食子儿茶素、没食子儿茶素没食子酸酯7种多酚成分被视为特征性标志成分,可用于鉴别普洱生茶贮藏时间。

    Abstract:

    This paper was to discriminate raw Pu-erh tea model from different storage times by the feature of polyphenols. The representative contents of 18 samples obtained from different storage times were analyzed by high performance liquid chromatography. Principal Component Analysis, Cluster Analysis and Partial Least Squares Discrimination Analysis model were applied to identify as the significant components which was visibly altered in the different storage of raw Pu-erh tea. The results showed that the catechin showed a gradually decreasing trend, the theaflavins showed a gradually increasing trend. Some components of flavonoid and flavonoid glycosides showed a downward trend while others showed an upward trend. The results of stoichiometric analysis showed that the raw Pu-erh tea samples from the same storage times could be aggregated, but the results of separation between the raw Pu-erh tea samples with different storage times were not good. It showed that obvious separation trend of the raw Pu-erh tea samples in the different storage of times by partial least squares discrimination analysis model. According to the Variable Importance Plot (VIP>1) in partial least square analysis model, combined with the content difference analysis, the result showed that gallic acid, kaempferol, theaflavin-3-gallic, epigallocatechin, epicatechin, gallocatechin and gallocatechin gallate had obviously change during the storage of the raw Pu-erh tea. To sum up, the result showed 7 components were used as signature by discrimination model.

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林 昕,王 丽,陈兴莲,邵金良,兰珊珊,魏茂琼,沙林杰,刘宏程.基于多酚类物质差异性判别普洱生茶的贮藏年限[J].中国食品学报,2021,21(10):240-246

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  • 在线发布日期: 2021-11-22
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