In order to understand the content and distribution of antibiotic resistance genes （ARGs） and intI1 in aquatic products， a quantitative real-time PCR method was established. Quantitative real-time PCR method was set up using recombinant plasmid containing the target genes as templates. And antibiotic resistance genes including tetA， sul2， blaPSE， cmlA， qnrS， aac（6'）-Ib and intI1 were quantify by established qPCR methods. Results indicated that all the detected genes could be detected in the commercial Macrobrachium， Carassius auratus， Pelteobagrus fulvidraco and Larimichthys crocea. The detection limit of blaPSE was 6.6×104 copies/μL， and the detection limits of other ARGs were between 11.5 and 202 copies/μL. Among the four kinds of aquatic products， the relative abundances of blaPSE were the highest， ranging from 1.17×10-4 to 5.96×10-3， followed by the relative abundances of aac（6'）-Ib ranging from 2.38×10-8 to 6.71×10-7. The obtained detection system can quantify 6 ARGs and intI1 gene in aquatic products simultaneously with the total bacterial DNA from the samples. The result could reveal the contaminated ARGs type and quantity of the commercial aquatic products， which is significant for controlling the ARGs pollution in aquatic products and ensuring the safety of aquatic food.