Aflatoxin is a secondary metabolite produced by Aspergillus flavus and Aspergillus parasite， which widely exist in various spoiled foods. It is stable， high toxic， and easy to cause cancer， which can cause disease in livestock and humans， seriously endangering the health of animals and consumers. Under improper storage conditions， foods are susceptible to mold infection to produce aflatoxin. Therefore， accurate and rapid aflatoxin detection methods are essential to ensure food safety and consumer safety. The immunoassay has the advantages of strong specificity， high sensitivity， safety and reliability， and has become one of the most competitive detection technologies. This article summarized the research progress of rapid detection technology for aflatoxin based on immunoassay in recent years， mainly including colorimetry， fluorometry， immunochromatography， electrochemical method， quartz crystal microbalance and microcantilever. The basic principle， performance， domestic and foreign research situation， drawbacks of these method were reviewed. It maybe provides a strategy and basis for the development of new ideas.