In order to explore the anti-oxidation and hypoglycemic activity of millet， the DPPH· scavenging capacity and total antioxidant capacity of four different extracts， extracts digestive solution and sediment digestive solution of millet were determined. The glucose uptake levels on PA-induced insulin-resistant HepG2 cells （IR-HepG2） were detected. The results showed that the water extract and ethanol extract had higher antioxidant activity， and their antioxidant capacity decreased after simulated digestion in vitro （the total antioxidant capacity decreased by 50.00% and 54.55%， respectively）. Unlike the antioxidant results， the water extract and the ethanol extract （20， 40 μg/mL） had no significant effect on the improvement of IR-HepG2 insulin resistance. The n-butanol extract （40， 80 μg/mL） and n-hexane extract （20， 40， 80 μg/mL） all significantly increased the glucose uptake of IR-HepG2. After digestion， the ethanol extract significantly improved insulin resistance， but n-butanol and n-hexane extracts had no effect on insulin resistance. The glucose uptake was enhanced by n-hexane sediment digestive solution， n-butanol sediment digestive solution， water sediment digestive solution and millet digestive solution. However， ethanol sediment digestive solution did not improve insulin resistance， indicating that the hypoglycemic effect of millet was mainly concentrated in ethanol extract.