There is a big difference in the hypoglycemic activity in vitro between polysaccharides by Inonotus obliquus from submerged fermentation and fruit body. In order to improve the activity of the former， this study explored the glucose consumption of HepG2 cells and insulin-resistant HepG2 cells added with extracellular polysaccharides from Inonotus obliquus by submerged fermentation under different stimulating factors. The results showed that polysaccharides obtained by adding stimulating factors could promote the glucose consumption of cells， and different stimulating factors had different effects of promoting hypoglycemic activity in vitro. To be specific， 160 μg/mL birch extract， 40 μg/mL VB6， 80 μg/mL VB1 and betulin group fermented polysaccharides could significantly increase the glucose consumption of IR-HepG2 cells for 48 h， and the effect was better than the non-stimulating factor group. Among them， thepromotion effect of VB6 was the most significant， which was 66.80% higher than that of the non-stimulating factor group， and significantly higher than that of the positive control group （P<0.01）. Therefore， the paper proved that the birch indeed contained stimulating factors that could promote the production of active polysaccharides from the fruit bodies of Inonotus obliquus， and further proved that the betulin， VB1 and VB6 in the birch extract could improve the hypoglycemic activity of the polysaccharide produced by the liquid submerged culture of Inonotus obliquus.