羊奶源产细菌素乳酸菌筛选、鉴定及益生特性研究
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国家自然科学基金项目(31801663);上海市自然科学基金项目(18ZR1449900);中央级公益性科研院所基本科研业务费专项资金(2016M02)


Screening, Identification and Probiotic Characteristics of Bacteriocin-producing Strains of Lactic Acid Bacteria from Goat Milk
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    摘要:

    以常见食源性致病菌(单增李斯特菌、金黄色葡萄球菌、蜡样芽孢杆菌)为指示菌,从鲜羊奶中筛选获得3株乳酸菌,并对其抑菌性质及益生特性进行了研究。结果显示:3株乳酸菌分别鉴定为粪肠球菌(命名为DH9003和DH9012)和乳酸乳球菌(命名为DH9011)。3株乳酸菌的抑菌产物具有细菌素的特征(耐高温和蛋白酶处理后失活)且在低质量浓度条件下(MIC50和4MIC50)可以阻止单增李斯特菌生长,其中2株粪肠球菌(DH9003和DH9012)产生的细菌素在高质量浓度(16MIC50)条件下可完全抑制单增李斯特菌生长。3株产细菌素乳酸菌均无溶血性,都对氨苄青霉素(50 mg/mL)和硫酸链霉素(100 mg/mL)敏感;在胆盐、模拟肠道和不同pH胃液(2.5,3.0,3.5)环境中均可存活;同时,3株乳酸菌均有较好的自聚集性,自聚范围为30%~55%,乳酸菌DH9003和DH9012与单增李斯特菌和大肠埃希氏菌的共聚集性相对较高,分别为52.6%、63.2%和68.5%、57.6%,而乳酸菌DH9011与单增李斯特菌的共聚集性为15.6%,与大肠埃希氏菌不聚集;3株乳酸菌均具有较高的β-半乳糖苷酶活力。上述试验结果表明:3株产细菌素乳酸菌的抑菌能力强,能够耐胆盐、人工肠胃液,有良好的黏附能力,安全可控,具有益生菌特质及应用价值。

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    In this study, three lactic acid bacteria (LAB) with antimicrobial activity against common food-borne pathogens (Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus) were obtained from goat milk, and their antimicrobial properties and potential probiotic characteristics were described. Those strains were identified as Enterococcus faecalis (termed as DH9003 and DH9012) and Lactococcus lactis (termed as DH9011), and their antimicrobial products showed typical bacteriocin traits, such as being of heat-stable and proteinase nature. Bacteriostatic activity of concentrated bacteriocin produced by those LAB was found at low mass concentration (MIC50 and 4MIC50), while complete inhibition activity against Lis. monocytogenes was found from two En. faecalis strains (DH9003 and DH9012) at high mass concentration (16MIC50). Besides of that, all those LAB were of no-hemolytic activity, sensitivity to ampicillin (50 mg/mL) and streptomycin sulfate (100 mg/mL), and resistance to bile, artificial simulated intestinal tract and gastric juice at different pH level (2.5, 3.0, 3.5), β-galactosidase activity. In addition, all those LAB present auto-aggregating phenotype with ranged from 30%-55%. DH9003 and DH9012 showed good co-aggregation ability with Lis. monocytogenes and E.coli (52.6%, 63.2% and 68.5%, 57.6%, respectively), while less co-aggregation ability between DH9011 and Lis. monocytogenes was obtained as 15.6%, and no co-aggregation with E.coli. The results in our study demonstrated that all those LAB were of strong antimicrobial activity, tolerance to bile and simulated gastrointestinal environments, adhesion ability, safe characteristics. Therefore, those LAB are considered as potential probiotic candidates with huge application values.

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杨珍珠;潘秭琪;迟海;陶乐仁.羊奶源产细菌素乳酸菌筛选、鉴定及益生特性研究[J].中国食品学报,2021,21(11):71-77

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  • 在线发布日期: 2021-12-17
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