For the purpose of studying the scientific design scope of fat-soluble vitamins A， D， E and K in infant formula milk powder for Chinese babies and providing reference for R & D personnel. Firstly， the test values from 11 different laboratories for the same batch of products were analyzed， the test deviations of vitamin A， D， E and K for formula design were determined to be 15%， 15%， 25% and 20%， respectively. Secondly， nutrient content in breast milk， adequate intakes， and regulation requirements for infant formula milk powder were compared and analyzed， the formula designing target values of vitamin A， D， E， and K were determined to be 60 μg RE/100 kcal， 2 μg/100 kcal， 0.6 mg α-TE/100 kcal， and 4.2 μg/100 kcal， respectively. Thirdly， reasonable nutrient design ranges were calculated by the determined target values of formula design， nutrient shelf life loss rate and detection deviation among different laboratories， and compared with tolerable upper intake level， the distribution of nutrients content of products that sold in China， meanwhile， the nutritional status of infants aged 0 to 6 months were taken into account. Finally， the scientific design range of fat-soluble vitamins A， D， E and K in Chinese infant formula milk powder was determined to be 100-120 μg RE/100 kcal， 3.3-4 μg/100 kcal， 1.2-2.5mg α-TE/100 kcal and 9.3-12 μg/100 kcal respectively.