微生物共培养对植物乳杆菌RX-8合成细菌素的诱导作用
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(1.北京食品营养与人类健康高精尖创新中心 北京市食品添加剂工程技术研究中心 北京工商大学 北京 100048;2.天津市和平区市场监督管理局 天津 300041)

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聂蓉(1997—),女,硕士生

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国家自然科学基金面上项目(31871772);


Induction of Plantaricin RX-8 from Lactobacillus plantarum RX-8 Production by Co-culture
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(1.Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University,Beijing 100048;2.Tianjin Heping District Market Supervision and Management Bureau,Tianjin 300041)

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    摘要:

    目的:植物乳杆菌RX-8分离自中国传统泡菜,可代谢合成Ⅱb类细菌素plantaricin EF。该细菌素抑菌谱广,加工应用特性良好,有作为天然食品生物防腐剂的巨大潜力,然而,其合成水平较低,工业化生产及应用严重受限。本研究探讨外源微生物共培养作为环境刺激因子,诱导菌株RX-8高效合成plantaricin EF的可行性。方法:基于菌株RX-8代谢产细菌素的现有常规纯培养条件及其主要影响因素,构建低产、不产细菌素的纯培养模型体系,分别添加16株不同发酵食品来源菌株进行共培养,通过测定共培养前、后代谢所产细菌素的抑菌活性,筛选最佳共培养诱导菌株及其条件。对比分析诱导菌株不同处理菌体细胞及发酵上清液的诱导作用,初步推测起诱导作用的物质类型。结果:添加谷物醋来源的枯草芽孢杆菌BS-15共培养,可在低产、不产及常规培养体系中均表现显著诱导作用(P<0.01)。该菌株与植物乳杆菌RX-8共培养的最佳接种比例是10∶1、接种浓度分别是108 CFU/mL和107 CFU/mL。推测诱导作用是由菌株BS-15分泌产生的蛋白类物质所引起。结论:确定存在的特定微生物可以诱导细菌素plantaricin EF高效分泌表达,微生物共培养可作为提高乳酸菌细菌素产量的重要技术手段。

    Abstract:

    Objective:Lactobacillus plantarum RX-8 was isolated from Chinese traditional pickles and shown to produce plantaricin EF,a genetically characterized inducible class IIb bacteriocin.It has broad spectrum antibacterial ability,good processing application characteristics and great potential application as a natural food biological preservative.However,plantaricin EF encounter various limits both in industrial fermentation and application because of its low synthesis.This work aimed at showing the effect of exogenous microorganisms act as an environmental stimulus activating plantaricin EF production by Lactobacillus plantarum RX-8.Method:Based on the existing conventional pure culture conditions and the main influencing factors of bacteriocin production by strain RX-8,two pure culture model systems with low and no bacteriocin production were established.Aliquots of the 16 strains from different fermented food sources were added to fresh MRS broth containing Ca.108 CFU/mL of an overnight culture of Lactobacillus plantarum RX-8.By comparing the bacteriocin antibacterial activity in co-culture and pure culture,the best co-culture induced strains and their conditions were screened and determined.To study the specific types of induction,the inducing activity of different treatments with living-cells and supernatants were tested.Results:When Bacillus subtilis BS-15 from grain vinegar was co-cultured with the strain RX-8,it showed significant induction ability (P<0.01).The best bacteriocin production was induced by co-cultivation as follows:the optimal inoculation ratio of Bacillus subtilis BS-15 and Lactobacillus plantarum RX-8 was 10:1,and the inoculation concentration was 108 CFU/mL and 107 CFU/mL respectively.It was speculated that the induction was caused by the protein substances secreted by Bacillus subtilis BS-15.Conclusion:Taken together,the results suggest that the presence of specific microorganisms act as environmental stimulus activating plantaricin EF production.Microbial co-cultivation can be used as an important technical means to increase the production of lactic acid bacteria.

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聂蓉,李雪,郜亚昆,刘国荣,王成涛,孙宝国.微生物共培养对植物乳杆菌RX-8合成细菌素的诱导作用[J].中国食品学报,2022,22(1):21-30

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  • 收稿日期:2021-01-08
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  • 在线发布日期: 2022-02-11
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