双孢菇边角料制备ACE抑制肽
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(甘肃农业大学食品科学与工程学院 兰州 730070)

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王睿(1996—),男,硕士生

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国家重点研发计划项目(2018YFD0400205)


Preparation of ACE Inhibitory Peptide from Scraps of Agaricus bisporus
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(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070)

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    摘要:

    以工厂生产的双孢菇菇柄、异形菇等边角料为原料,利用碱溶酸沉法提取双孢菇蛋白,超声波处理后,分别选用胰蛋白酶、碱性蛋白酶和复合蛋白酶对其进行酶解,通过测定蛋白水解度和ACE抑制率确定最佳的蛋白酶种类。采用响应面设计方法,优化蛋白酶酶解工艺条件,以期获得更高ACE抑制率的活性肽。结果表明,碱性蛋白酶酶解双孢菇蛋白产生的ACE抑制率最大,其最优酶解工艺参数为:碱性蛋白酶添加量6.25 mg/100 mL,酶解温度40.47 ℃,酶解时间92.97 min,酶解pH值8.04,此时ACE抑制率可达43.75%,在该条件下的验证试验测得水解液的ACE抑制率为43.4%,与预测值相近。经真空冷冻干燥,制得具有抑制ACE活性的肽样品,得率为24.36 mg/g,工业化生产前景良好,可为双孢菇产业边角料高值利用提供新途径。

    Abstract:

    Using the scraps such as the stalks of bisporus mushrooms and irregular mushrooms produced in the factory as raw materials,the protein of Agaricus bisporus is extracted by the alkaline solution and acid precipitation method,after ultrasonic treatment,trypsin,alkaline protease and composite protease were selected to hydrolyze the protein.By measuring the degree of protein hydrolysis and ACE inhibition rate,the best kind of protease was determined.In order to obtain the active peptide with higher ACE inhibition rate,the enzymatic hydrolysis conditions were optimized by using response surface methodology(RSM).The results showed that the ACE inhibition rate of A.bisporus protein hydrolyzed by alkaline protease was the highest.The optimal process parameters were as follows:the addition amount of alkaline protease was 6.25 mg/100 mL,the hydrolysis temperature was 40.47 ℃,the hydrolysis time was 92.97 min,and the hydrolysis pH was 8.04,the ACE inhibition rate can reach 43.75%.A verification test was performed under the condition,and the ACE inhibition rate of the hydrolyzed solution was 43.4%,which was close to the predicted value.After ultrafiltration and vacuum freeze-drying,the peptide sample with potential ACE inhibitory activity was prepared,and the yield was 24.36 mg/g.The industrial production prospect was good,which could provide a new way for the high value utilization of the industrial scraps of A.bisporus.

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王睿,贠建民,何奎,武淑娟,毕阳,赵风云.双孢菇边角料制备ACE抑制肽[J].中国食品学报,2022,22(1):125-134

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  • 收稿日期:2021-01-19
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  • 在线发布日期: 2022-02-11
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