固相萃取法联用UPLC-Triple-TOF/MS法鉴定花菜中自由和结合态植物甾醇
作者:
作者单位:

(1.浙江工业大学食品科学与工程学院 杭州 310014;2.中国轻工业食品大分子资源加工技术研究重点实验室(浙江工业大学) 杭州 310014;3.浙江华才检测技术有限公司 浙江绍兴 311800)

作者简介:

冯思敏(1989—),男,博士生,副研究员

通讯作者:

中图分类号:

基金项目:

浙江省重点研发计划项目(2019C02070,2021C02019);国家自然科学基金青年科学基金项目(31801536)


Identification of Free and Bound Phytosterols in Cauliflower by Solid Phase Extraction Combined with UPLC-Triple-TOF/MS
Author:
Affiliation:

(1.College of Food Science and Engineering,Zhejiang University of Technology,Hangzhou 310014;2.Key Laboratory of Processing Technology of Food Macromolecule Resources in China Light Industry(Zhejiang University of Technology),Hangzhou 310014;3.Zhejiang Huacai Testing Technology Co.LTD,Shaoxing 311800,Zhejiang)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为探明花菜中植物甾醇的组成,采用固相萃取法(SPE)分离4种结合状态的甾醇,然后采用超高效液相色谱串联三重四极杆飞行时间质谱(UPLC-Triple-TOF/MS)法鉴定花菜中植物甾醇的种类,分析其结构。结果表明,花菜中含有的游离甾醇(FS)有豆甾醇、菜油甾醇、羽扇豆醇、β-谷甾醇;甾醇酯(SE)有菜油甾醇亚油酸酯、豆甾醇油酸酯、β-谷甾醇油酸酯;甾醇糖苷(SG)有菜油甾醇葡萄糖苷、β-谷甾醇葡萄糖苷;酰化甾醇糖苷(ASG)有菜油甾醇葡萄糖苷棕榈油酸酯、菜油甾醇葡萄糖苷二十碳烯酸酯、豆甾醇葡萄糖苷亚麻酸酯、β-谷甾醇葡萄糖苷二十碳烯酸酯、β-谷甾醇葡萄糖苷亚麻酸酯。

    Abstract:

    Phytosterols have been widely concerned for their efficacy in lowering cholesterol,anti-tumor,anti-inflammatory and anti-oxidation.In order to find out the composition of phytosterols in cauliflower,both free and three kinds of conjugated phytosterols were separated by solid phase extraction,and then the species and structure of phytosterols in cauliflower were identified and analyzed by UPLC-Triple-TOF/MS.The results showed that the free phytosterol(FS) in cauliflower were stigmasterol,campesterol,lupeol and β-sitosterol. Campesterol linoleate,stigmasterol oleate and β-sitosterol oleate were identified as three phytosterol esters(SE).Campesterol glucoside and β-sitosterol glucoside were identified as phytosterol glycosides (SG).Acylated sterol glycosides (ASG) include campesterol glucoside palm oleyl ester,campesterol glucoside eicosapentaenoic acid ester,stigmasterol glucoside linolenic acid ester,β-sitosterol glucoside eicosapentaenoic acid ester and β-sitosterol glucoside linolenic acid ester.

    参考文献
    相似文献
    引证文献
引用本文

冯思敏,吴思杰,江佳萍,廖杰,孙培龙,邵平.固相萃取法联用UPLC-Triple-TOF/MS法鉴定花菜中自由和结合态植物甾醇[J].中国食品学报,2022,22(1):238-250

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-01-17
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2022-02-11
  • 出版日期:
文章二维码
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知