骆驼乳对小鼠酒精性肝损伤的保护作用
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(1.内蒙古农业大学 乳品生物技术与工程教育部重点实验室 呼和浩特 010018;2.内蒙古骆驼研究院 内蒙古阿拉善 750306)

作者简介:

白路平(1994—),女,硕士生

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基金项目:

国家重点研发计划项目(2020YFE0203300);内蒙古自治区科技成果转化专项(AMCG201911);高层次人才引进科研启动项目(NDYB2017-28);双一流学科创新团队建设项目(NDSC2018-14);内蒙古自然科学基金项目(2018BS03017)


Protective Effect of Camel Milk on Alcoholic Liver Injury in Mice
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(1.Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education,Inner Mongolia Agriculture University, Hohhot 010018;2.Inner Mongolia Institute of Camel Research, Alxa 750306, Inner Mongolia)

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    摘要:

    目的:研究骆驼乳对Lieber-DeCarli液体饲料诱导的小鼠酒精性肝损伤的保护作用。方法:将40只雄性C57BL/6NCr小鼠随机分为对照组(Con)、模型组(Et)、骆驼乳高剂量组(EtCM_H,3 g/kg)、骆驼乳低剂量组(EtCM_L,1.5 g/kg)和阳性对照组(美他多辛,300 mg/kg)。试验8周,前4周仅饲喂专用饲料,不灌胃;后4周饲喂方式不变,灌胃乳或美他多辛,每日灌胃1次。灌胃结束后,按照5 g/kg剂量一次性灌胃31.5%酒精溶液,建立NIAAA模型。测定肝脏抗氧化、抗炎指标、细胞凋亡标志物活性、血清转氨酶活性,HE染色观察肝组织病理学变化,油红O染色观察肝脏脂质蓄积程度,TUNEL染色法检测肝细胞凋亡情况。结果:骆驼乳能显著降低肝损伤小鼠血清谷丙转氨酶和谷草转氨酶活力,降低小鼠肝组织丙二醛、甘油三酯、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)含量和半胱天冬蛋白酶-3活性,提高小鼠肝组织超氧化物歧化酶活力,提高还原型谷胱甘肽和白介素-10(IL-10)含量,减少肝脏内脂质蓄积,减轻肝细胞水肿,拮抗肝细胞凋亡。结论:骆驼乳对Lieber-DeCarli液体饲料诱导的小鼠酒精性肝损伤具有保护作用,呈现剂量依赖性特点,高剂量优于低剂量。骆驼乳可能通过提高肝细胞抗氧化能力与抗炎因子水平,促进脂肪转运,进而使肝细胞结构和功能逐渐修复来实现这种保护作用。

    Abstract:

    Objectives: To study the protective effect of camel milk on alcoholic liver injury in mice induced by Lieber-DeCarli liquid diet. Methods: 40 male C57BL/6NCr mice were randomly divided into control group(Con), model group (Et), camel milk high-dose group (EtCM_H, 3 g/kg), camel milk low-dose group (EtCM_L, 1.5 g/kg) and positive control group (Metadoxine, 300 mg/kg). The experiment was conducted for a total of 8 weeks. The first 4 weeks were only fed with special feed without gavage. After 4 weeks, the feeding method was unchanged, with milk or metadoxine by gavage, once a day. After intragastric administration, the NIAAA model was established by intragastric administration of 31.5% alcohol solution at a dose of 5 g/kg. The indicators of liver antioxidant and anti-inflammatory, apoptosis markers contents, serum transaminase activity were measured. HE staining was used to observe liver histopathological changes. Oil Red O staining was used to observe liver lipid accumulation. TUNEL staining was used to detect liver cell apoptosis. Results: Camel milk could significantly reduce the activities of serum alanine aminotransferase and aspartate aminotransferase in mice with liver injury, and reduce liver tissue malondialdehyde, triglycerides, and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) content and Caspase-3 activity, improve the activity of superoxide dismutase in mouse liver tissue, reduce glutathione and interleukin-10 (IL-10) content, reduce lipid accumulation in the liver, reduce liver cell edema, and antagonize liver cell apoptosis. Conclusion: Camel milk has a protective effect on alcoholic liver injury induced by Lieber-DeCarli liquid diet in mice, and presents a dose-dependent characteristic. High dose is better than low dose. Camel milk may improve liver cell antioxidant capacity and increase the content of inflammatory factors to promote fat transport, and then restore the structure and function of liver cells to achieve this protective effect.

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白路平,乔向宇,吉日木图,明亮.骆驼乳对小鼠酒精性肝损伤的保护作用[J].中国食品学报,2022,22(2):140-149

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  • 收稿日期:2021-02-20
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  • 在线发布日期: 2022-03-03
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