酿酒酵母Y3401产己酸乙酯发酵条件的优化
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(1.北京工商大学 北京食品营养与人类健康高精尖创新中心 北京 100048;2.北京工商大学 北京市食品添加剂工程技术研究中心 北京 100048;3.北京工商大学食品学院 北京 100048;4.安琪酵母股份有限公司 酿造与生物能源研究所 湖北宜昌 334003)

作者简介:

刘朋肖(1994—),女,硕士

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国家自然科学基金青年科学基金项目(31701592)


Optimization of Fermentation Conditions for Ethyl Caproate Production from Saccharomyces cerevisiae Y3401
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(1.Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology & Business University, Beijing 100048;2.Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business, Beijing 100048;3.School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048;4.Institute of Brewing and Bioenergy, Angel Yeast Co. Ltd., Yichang 334003, Hubei)

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    摘要:

    以酿酒酵母Y3401为研究对象,对该菌株发酵产己酸乙酯条件进行优化。首先,通过单因素对其培养基条件(pH值和糖度)及诱导条件(温度、转速、接种量、乙醇添加量、己酸添加量、前体添加时机和诱导时间)进行优化,然后通过Plackett-Burman试验,筛选出5个显著影响酿酒酵母Y3401产己酸乙酯的因素,再通过最陡爬坡试验确定最大响应区域,最后采用Box-Behnken试验设计及响应面分析,确定其最优产己酸乙酯条件为:糖度14 Brix、初始pH 7、温度25 ℃、转速180 r/min、接种量3.5%、乙醇添加量8%、己酸添加量0.059%、前体添加时机30 h、诱导时间31 h。在此培养条件下己酸乙酯产量达10.1 mg/L。本研究结果有助于酿酒酵母Y3401更好地应用于白酒发酵中。

    Abstract:

    Saccharomyces cerevisiae Y3401 with high-yield for ethyl caproate was selected, and the fermentation conditions of ethyl caproate from it were optimized. Firstly, the medium conditions (including initial pH value and sugar content) and induction conditions (including temperature, rotation speed, inoculation amount, ethanol addition amoun, caproic acid addition amount, pre-induced period, and post-induced period) were optimized by single factor design. Then, five significant factors for ethyl caproate production were screened by Plackett-Burman design, and the maximum response area was determined by the steepest ascent design. Finally, the optimal fermentation conditions of Y3401 for producing ethyl caproate were obtained by response surface methodology. The optimal conditions were as follows: sugar content 14 Brix, initial pH 7, inoculum size of 3.5%, ethanol content 8%, caproic acid content 0.059%, pre-induced period 30 h, and post-induced period 31 h incubated at 25 ℃ with 180 r/min. Under the condition, the yield of ethyl caproate reached 10.1 mg/L. These results are helpful for the application of S. cerevisiae Y3401 in Baijiu brewing.

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刘朋肖,常煦,成柳洁,丁泽,龚艺,李秀婷,范光森.酿酒酵母Y3401产己酸乙酯发酵条件的优化[J].中国食品学报,2022,22(2):178-189

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  • 收稿日期:2021-02-07
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  • 在线发布日期: 2022-03-03
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