骆驼乳对葡聚糖硫酸钠诱导小鼠结肠炎的保护作用
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(1.内蒙古农业大学 乳品生物技术与工程教育部重点实验室 呼和浩特 0100182.内蒙古骆驼研究院 内蒙古阿拉善 750306)

作者简介:

郭坤杰(1995—),女,硕士生

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基金项目:

内蒙古自治区自然科学基金项目(2020BS03040)


Preventive Effects of Camel Milk on Dextran Sulfate Sodium-induced Ulcerative Colitis in Mice
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(1.Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education,Inner Mongolia Agricultural University, Hohhot 010018;2.Inner Mongolia Camel Research Institute, Alxa 750306, Inner Mongolia)

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    摘要:

    探讨骆驼乳(CM)对葡聚糖硫酸钠(DSS)诱导小鼠结肠炎的保护作用,为结肠炎的研究和治疗提供新的切入点。小鼠自由饮用含2.5% DSS的水,造模,连续7 d。将60只C57BL/6小鼠随机分成4组:空白组、模型组、CM组(10 mL/kg)和CM+DSS组。通过体重变化、疾病活动指数(DAI)评分、病理学评分和免疫组织化学评估CM对结肠炎小鼠的作用。采用ELISA和实时荧光定量PCR检测血清炎性细胞因子水平和结肠髓过氧化物酶(MPO)活性,进一步探究CM对炎症的影响。结果显示,与模型组相比,CM干预后能显著降低DSS诱导结肠炎小鼠的体重损失和DAI评分(P<0.05),显著减少结肠MPO活性和血清炎性细胞因子(IL-1β、IL-6和IL-10)水平(P<0.05),并能缓解结肠缩短症状(P<0.05),保护结肠组织结构。CM具有抑制中性粒细胞浸润,降低炎症和保护结肠组织结构的作用,对DSS诱导的小鼠结肠炎损伤有一定的保护作用。

    Abstract:

    The aim of our study was to investigate the protective role of camel milk(CM) on dextran sulfate sodium (DSS)-induced colitis in mice, which might provide a new entry point for the research and treatment of colitis. In this study, ulcerative colitis was induced by oral administration of 2.5% DSS for 7 days in drinking water. A total of 60 male C57BL/6 mice were randomly divided into 4 groups: control group, model group, CM group (10 mL/kg) and CM+DSS group. The protective effect of CM was assessed by weight change, disease activity index(DAI), histopathological scores and immunochemistry. Detect the level of inflammatory cytokines and myeloperoxidase (MPO) activity in colon and serum by ELISA and real-time fluorescent quantitative PCR to further reflect the influence of camel milk on inflammation. Compared with the model group, CM supplement decreased weight loss and DAI (P>0.05), also significantly reduced the levels of MPO activity and inflammatory cytokine(IL-1β, IL-6 and IL-10) levels in serum and colon tissue (P<0.05). Meanwhile, CM can relieve the colon shorten symptoms(P<0.05) and protect colon tissue structure. Camel milk has the effects of inhibiting neutrophil infiltration, reducing inflammation and protecting the structure of colon tissue, and has a certain protective effect on DSS-induced colitis damage in mice.

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郭坤杰,何静,吉日木图.骆驼乳对葡聚糖硫酸钠诱导小鼠结肠炎的保护作用[J].中国食品学报,2022,22(3):62-71

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  • 收稿日期:2021-03-18
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  • 在线发布日期: 2022-04-13
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