The aim of this study was to prepare a new kind of structured lipids consisting of soybean oil and lauric acid aimed at studying their effects on lipid metabolism and obesity inhibition of C57BL/6J mice， thus providing a theoretical basis for the development of structured lipids that can effectively control obesity and reduce related diseases. The MLM-type (medium-long-medium chain) SLs were synthesized by enzymatic interesterification from soybean oil and lauric acid. Twenty healthy C57BL/6J mice were selected and divided into four groups by feeding different feeds， ordinary feed + normal saline group (DF + NS)， high-fat feed + structural lipid group (HF + LaSLs)， ordinary feed + soybean oil group (DF + SO)， and ordinary feed + structural lipid group (DF + LaSLs). The changes in body weight of mice after feeding for 30 days were observed. Some values including serum triglycerides， cholesterol， high-density cholesterol， low-density cholesterol and organ index were detected to analyze and evaluate the influence of soybean oil lauric acid SLs on improving lipid metabolism and preventing obesity. Studies showed that the addition of soybean oil-lauric acid SLs can affect the absorption of mice receiving a standard diet or a high-fat diet， thereby effectively preventing the weight gain of mice. The levels of triglycerides， total cholesterol， HDL-C and LDL-C in the serum were effectively regulated， and there were no significant change in the activity of aminotransferase (ALT) and aspart Aminotransferase (AST). Additionally， the results indicated that the new structured lipids obtained had good potential for controlling obesity and would not produce side effects.