基于非靶向代谢组学的春闺与铁观音乌龙茶品质研究
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(福建省农业科学院茶叶研究所 福州 350012)

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余文权(1972—),男,博士,教授级高级农艺师

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基金项目:

国家茶叶产业技术体系项目(CARS-19);福建省属公益类科研院所基本科研专项(2018R1012-6)


Studies on the Quality of Chungui and Tieguanyin Oolong Tea Based on Non-targeted Metabonomics
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(Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350012)

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    摘要:

    春闺(CG)是单株育种法育成的适制乌龙茶高香新品种。为探明春闺乌龙茶香气滋味特征品质及形成原理,以春闺和铁观音鲜叶为原料分别制成春闺乌龙茶和铁观音乌龙茶,采用顶空固相微萃取(HS-SPME)结合全二维气相色谱-飞行时间质谱(GC×GC-TOF-MS)和超高效液相色谱串联四极杆飞行时间质谱(UHPLC-Q-TOF-MS)非靶向代谢组学技术,分析春闺闽南乌龙茶挥发性与非挥发性代谢组分分布特点及其与铁观音乌龙茶的差异。结果表明:春闺乌龙茶挥发性香气组分整体与铁观音的接近,相对含量最高的组分为吲哚,占10.34%,吲哚、橙花叔醇和茉莉内酯等重要挥发性组分显著高于铁观音乌龙茶。春闺乌龙茶非挥发性组分整体与铁观音乌龙茶差别较大,春闺乌龙茶EC、ECG和原矢车菊素B1等儿茶素单体或二聚体显著低于铁观音乌龙茶,与儿茶素组分具有共同前体的黄酮醇或黄酮糖苷类物质、γ-氨基丁酸、谷氨酸和茶氨酸葡萄糖苷均显著高于铁观音乌龙茶,色氨酸相对含量显著低于铁观音乌龙茶,这可能与春闺乌龙茶中吲哚富集有关。这些共同构成春闺乌龙茶花香持久、茶汤醇爽的品质特征。

    Abstract:

    Chungui (CG) variety is a new oolong tea suitable variety bred by using single plant breeding method. To explore the characteristic aroma and taste quality of CG oolong tea, the HS-SPME coupled with GC×GC-TOF-MS and UHPLC-Q-TOF-MS non targeted metabonomics technique were used to investigate the volatile and non-volatile components of CG oolong tea and Tieguanyin(TGY) oolong tea. The results showed that the volatile aroma components were close to TGY oolong tea. Indole accounted for 10.34% of the total volatile components in CG oolong tea, and the important volatile components such as indoles, nerolidol and jasmine lactone were significantly higher than TGY oolong tea. CG non-volatile components were showed grate difference between TGY oolong tea. The monomer or dimer catechins such as EC, ECG and procyanidin B1 in CG oolong tea were significantly lower than that in TGY oolong tea. However, flavonols or flavonoid glycosides which share the same precursors with catechins, γ-aminobutyric acid, glutamate and theanine glycosidase were significantly higher than that of TGY oolong tea. The relative contents of tryptophan was significantly lower than TGY oolong tea, this may be related to indole concentration in spring best oolong tea. These metabolites constitute the characteristics of lasting flower fragrance and mellow tea soup of CG oolong tea.

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余文权,李鑫磊,钟秋生,林郑和,单睿阳,陈志辉,陈常颂.基于非靶向代谢组学的春闺与铁观音乌龙茶品质研究[J].中国食品学报,2022,22(5):300-308

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  • 收稿日期:2021-05-04
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  • 在线发布日期: 2022-06-15
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