Objective: In this study， endophytic fungi from wild Glycyrrhiza uralensis Fisch were isolated and the antibacterial and antioxidant activities of their secondary metabolites were evaluated. Method: Using fresh wild licorice as material， the different tissue parts of licorice were treated by tissue cutting method， and then the endophytic fungi were separated and purified with 9 different media and carry out molecular biological identification. The antimicrobial activities of their metabolites against five tested strains was evaluated by paper diffusion method. The antioxidant activity of the metabolites was evaluated by DPPH and ·OH radical scavenging capacity. Results: 128 strains of fungi were isolated from the plant licorice， which belonged to 5 different genera through molecular biology identification. YS-6 fermentation broth showed certain antibacterial activity against Staphylococcus aureus. The DPPH free radical scavenging rate of GS-6a fermentation broth was 97.54%， and the ·OH free radical scavenging rate of GS-6a mycelium was 98.83%. Conclusion: The resources of endophytic fungi isolated from licorice are abundant and diverse， which are good sources for screening antibacterial and antioxidant substances.