The formation kinetics of 5-hydroxymethylfurfural was investigated in sugar-peptide model systems (glucose-glutamate dipeptide). In this paper， glucose-glutamate dipeptide and 5-hydroxymethylfurfural(5-HMF) contents were indicated by kinetic analysis method. The kinetics analysis showed that the consumption of glucose and glutamate dipeptide were all followed first-order kinetics in glucose-glutamate dipeptide models at 90-110 ℃ (0-6 h). And the degration rate of reactants increased gradually with temperature. HMF formation followed zero-order kinetic at 90-95 ℃(0-6 h) and followed first-order kinetics at 100-110 ℃(0-6 h) in glucose-glutamate dipeptide models.