Objective: To study hypoglycemic activity of diosmetin on type Ⅱ diabetic mice. Methods: Activities of four phenolic compounds in Chrysanthemum morifolium including diosmetin， isochlorogenic acid C， luteolin and morin， were analyzed using molecular docking and α-amylase， α-glucosidase experiments. Type Ⅱ diabetic mice model was developed using high sugar and fat feed combined with streptozotocin(STZ) method. Effects of diosmetin on carbohydrate metabolism， lipid metabolism and oxidative stress in mice were investigated using homeostasis model assessment of insulin resistance (HOMA-IR)， total cholesterol (TC) and glutathione peroxidase (GSH-Px) methods. Results: Diosmetin effectively alleviates the symptoms of diabetic mice such as polyphagia， polydipsia and low body weight， significantly reduces fasting blood glucose(FBG)， insulin (I) content and insulin resistance index (P<0.05)， decreases content of glycosylated serum protein(GSP)， and relieve the abnormity of oral glucose tolerance test (OGTT). Besides， diosmetin reduces contents of total triglycerides (TG)， TC and low density lipoprotein cholesterol (LDL-C) in the serum， and increase content of high density lipoprotein cholesterol (HDL-C). Diosmetin also improves total anti-oxidation capacity (T-AOC) and activities of superoxide dismutase(SOD) and GSH-Px， reduces malondialdehyde(MDA) content， and significantly reduces activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (P<0.05). Conclusion: Diosmetin exhibits hypoglycemic activity on type II diabetic mice， and possesses mitigating effects on oxidative stress and lipid metabolism disorders caused by diabetes.