During the winemaking process， excessive copper has the effect of inhibiting fermentation， causing oxidative browning of wine. Metallothionein is a type of protein with high cysteine content and low molecular weight that can be induced by metals. The use of cell surface display technology to express that metallothionein can largely avoid the problem of metal ions transmembrane and reduce the damage of heavy metals to cells. In this study， the metallothionein gene CUP1 from Saccharomyces cerevisiae 288c was displayed on the surface of S. cerevisiae cells， and a diploid strain with higher adsorption capacity for copper ions in wine was successfully obtained. The research can provide new ideas for solving the problem of copper in wine and lay a theoretical foundation for the industrial application of CUP1 metallothionein whole-cell adsorbent.