There is an information exchange mechanism among microorganisms that lead to the deterioration of aquatic products， called "quorum sensing"(QS). The quorum sensing system of Gram-negative bacteria mainly uses N-acyl homoserine lactone as a signal molecule to indirectly regulate the spoilage characteristics of some spoilage bacteria in aquatic products. Therefore， the spoilage of aquatic products can be inhibited by quenching quorum sensing. As a quorum sensing inhibitor， quorum sensing quenching enzyme has been identified in many bacteria. In order to explore the potential function of pf-1240 gene expression product of Pseudomonas fluorescens (PF08)， gene cloning and bioinformatics analysis were used to study it. The results showed that the pf-1240 gene of Pseudomonas fluorescens PF08 might express a quorum sensing quenching enzyme， which was 65.82% similar to the second homoserine lactone acylase QuiP protein of Pseudomonas aeruginosa. Sequence analysis showed that pf-1240 gene encoded 795 amino acids. The protein molecular formula was C3867H6010N1084O1160S19， the relative molecular weight was 86 855.96， the theoretical isoelectric point (pI) was 7.81， and the instability coefficient was 35.29. According to the conserved domain analysis， the protein contains a complete domain of penicillin acylase family， and many enzymes in this family have been proved to have quorum sensing quenching enzyme function， which can decompose quorum sensing signal molecule N-acyl homoserine lactone (AHL). It is speculated that the protein also has similar function. In this study， the physical and chemical properties and structure of the protein were predicted by bioinformatics technology， so as to understand the properties and functions of quorum sensing quenching enzyme of Pseudomonas fluorescens.