Objective: To investigate the protective mechanism of dry powder of sea cucumber cooking liquid on acute liver injury mice， which will provide evidences in developing function food for liver protection. Methods: Acute liver injury models established by intraperitoneal injection of CCl4 corn oil solution with a volume fraction of 5% in mice. The activities of antioxidant enzyme and the contents of oxidation product were determined in serum and liver. Liver tissue was stained with H & E and immunohistochemistry for observing the of SCCL on mice liver histopathology. Expression of Nrf2， Keap-1， HO-1， NQO-1 mRNA were measured by qRT-PCR. Results: Compared with model group， the groups of SCCL， antioxidant enzyme activities significantly increased and oxidation product contents were declined significantly in serum and liver； the liver histomorphology was improved； expression levels of Nrf2， Keap-1， HO-1， NQO-1 were improved in the SCCL groups. Conclusion: The SCCL enhanced the antioxidant activity， ameliorateorganism damage by oxidative stress， effectively protect the acute liver injury by CCl4 in mice.