The establishment of nutritional quality evaluation system of honeysuckle is of great significance for screening and exploring excellent resources of honeysuckle， and comprehensive utilization of its nutritional and medical values. 138 honeysuckle varieties from different habitats were used as samples in this research. Quantitative analysis of chlorogenic acid， rutin， luteoloside， isochlorogenic acid A， isochlorogenic acid C， luteolin were performed by high performance liquid chromatography. The content of total flavonoids was determined by UV spectrophotometry. The comprehensive evaluation function of nutritional quality of honeysuckle was established by entropy weight method， grey correlation analysis and principal component analysis (PCA)， and the comprehensive evaluation standard of nutritional quality of honeysuckle was established by probability distribution. The results demonstrated that there were differences in the contents of seven nutrients among 138 tested materials， and the coefficient of variation was 8.32%-71.23%. All the seven nutrients were affected by the environmentbut different in degrees. Correlation analysis indicated that luteolin was positively correlated with chlorogenic acid and rutin. There was a significant positive correlation between isochlorogenic acid A and isochlorogenic acid C. Luteolin was negatively correlated with isochlorogenic acid A and isochlorogenic acid C. The total flavonoids showed a significant positive correlation with isochlorogenic acid A and isochlorogenic acid C， and a significant negative correlation with luteolin. PCA simplified seven nutritional components into three principal component factors. PC1 includes isochlorogenic acid A， isochlorogenic acid C and luteolin. PC2 includes chlorogenic acid and luteolin. PC3 includes rutin and total flavonoids. The contribution rates of the three principal components were 44.339%， 23.930% and 18.382% respectively， and the cumulative contribution rate was 86.651%. On this basis， the comprehensive evaluation function of nutritional quality of honeysuckle and the six grade standard of nutritional quality of honeysuckle were established. Comprehensive analysis of the nutritional quality of honeysuckle resources by using the evaluation system can get more objective nutritional evaluation results， and provide a basis for screening the best nutritional quality of honeysuckle resources.