In order to understand the diversity of fungal flora in different varieties of blueberries and clarify their respective dominant bacterial groups， this paper used high-throughput sequencing technology to analyze the gene sequences of ITS1 region in Hokuriku， Lanjin， Lexie， O'Neill blueberries， and compared the differences in microbial community structure and diversity from the surface of different blueberries. The results showed that the main dominant genus of blueberry were Cladosporium and Alternaria； and the dominant species were Cladosporium_chasmanthicola， Alternaria_alternata， Cladosporium_cladosporioides. The Simpson index showed that the abundance and diversity of the microbial community from high to low were Lexie， Hokuriku， O'Neill， and Blue Gold. There was a certain clustering relationship between the microbial community structure and varieties on the surface of different blueberries: Hokuriku and Lexie community structures were relatively similar， while the microbial communities of the other samples were quite different， and the relative abundance of the main colonies was significantly different， Cladosporium， Alternaria， and Cladosporium were the main different species. This study preliminarily defined the diversity of fungal flora in different varieties of blueberries， and provided certain theoretical support for ensuring the quality and safety of blueberries during storage.