Aeromonas sobria is a common spoilage bacteria in aquatic products， and its spoilage mechanism is closely related to quorum sensing. Therefore， the active substances with quenching quorum sensing activity to control A. sobria spoilage ability are being found. In this study， Pediococcus pentosaceus YF-8 could degrade N-acyl homoserine lactone signal molecule of A. sobria. Its inhibition effect on virulence factors， biofilm and quorum sensing regulated genes of A. sobria was studied. The results showed that the crude extracts of YF-8 with sub-minimum inhibitory concentration (2.0， 4.0 mg/mL and 6.0 mg/mL) had inhibition effect on the protease， extracellular polysaccharide， siderosin， hemolysis， and swimming capacity of A. sobria， and the inhibition rates reached 9.6%-32.72%， 17.91%-58.09%， 19.04%-44.35%， 19.21%-51.49% and 51.61%-93.55%， respectively. Meanwhile， the inhibition rate was concentration-dependent. In addition， the inhibition rate and clearance rate of YF-8 crude extracts with the three sub-MICs on A. sobria's biofilm reached 31.28%-66.64% and 6.7%-29.88%， and the thickness of biofilm became thin and the bacteria were sparse and loose. At the same time， the 6.0 mg/mL YF-8 crude extracts down-regulated expression of the LuxI and LuxR genes regulated quorum sensing of A. sobria， and the inhibiton rate reached 69.48% and 80.76%. This study will provide a new idea for controlling the corruption and disease of aquatic products caused by A. sobria.