Objective: To study the protective effects of Lactobacillus plantarum Lp2 on acute liver injury in mice. Methods: The effect and mechanism of L. plantarum Lp2 isolated from traditional Chinese fermented foods on improving lipopolysaccharide(LPS) induced liver inflammation in mice were studied. Male KM mice were randomly assigned into three groups: Control group， model group(LPS group)， and intervention group (LPS + Lp2 group). The mice in the LPS+Lp2 group were supplemented with L. plantarum Lp2 every day. After 4 weeks， the mice in the LPS group and the LPS+Lp2 group were injected intraperitoneally with LPS to construct acute inflammation model. The liver injury of LPS-induced mice was observed through the evaluation of biochemical indexes， liver histopathology， mRNA and protein expression level. Result: L. plantarum Lp2 treatment reduced significantly the levels of aspartate transaminase (AST)， alanine aminotransferase (ALT)， tumor necrosis factor-α (TNF-α)，interleukin-1β (IL-1β)， interleukin-6 (IL-6)， nitric oxide (NO)， cyclooxygenase-2 (COX-2)， malondialdehyde (MDA)， myeloperoxidase (MPO) and LPS in serum， liver and ileum tissue of mice with acute inflammation induced by LPS， and increased the levels of superoxide dismutase (SOD)， glutathione peroxidase (GSH-Px) and catalase(CAT). RT-PCR and western-blotting analysis showed that L. plantarum Lp2 pretreatment reduced ileum protein expression of ZO-1， Occludin and Claudin-1. Conclusion: L. plantarum Lp2 ameliorates LPS-induced acute liver disease by improving intestinal barrier integrity， inhibiting oxidative stress and attenuating inflammation.