The effect of sea cucumber peptides(SCP) on the wound healing of rats was investigated by establishing an excisional skin wound model in rats. Then， the anti-gastric ulcer was evaluated using an animal model of ulcerative lesions induced by acetic acid， and then the mice were gavaged with different doses of SCP(0.5 mg/g and 2 mg/g). The effect of antioxidant factors， inflammation cytokines， and defensive factor levels in the gastric tissue homogenate of rats were analyzed for the bioactivities of SCP. The results showed that the wound healing rate of the SCP group was significantly higher than control group from the 2th day after modeling and the wound had almost healed up after 12 days. Compared to the model group， the ulcer areaof SCP-treated rats was significantly decreased， and the inhibition rate reached 41.45% and 60.41%. The pathological findings suggested that mucosal regeneration， and inflammatory cell infiltration were decreased. The activity of superoxide dismutase (SOD) and glutathione peroxide dismutase (GSH-Px) was increased by 3.80%， 4.85% and 12.59%， 23.54%， respectively， and the content of malondialdehyde (MDA) was decreased by 59.40%， 45.24%， respectively. Furthermore， the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was decreased by 12.37%， 19.63% and 8.64%， 23.43%， respectively. In addition， the levels of nitric oxide (NO)， prostaglandin E2 (PGE2) and epidermal growth factor (EGF) was decreased by 12.80%， 16.95% and 14.06%， 27.44% and 25.08%， 63.76%， respectively. Therefore，the gastroprotective activity of sea cucumber peptides faction could be attributed to the increment of antioxidant， anti-inflammation and defense capabilities of gastric mucosa.