In this study， the optimal medium composition and static culture conditions of Lactiplantibacillus plantarum NCU137 were determined by single factor experiment， Plackett-Burman， steepest climb and central composite design using the number of viable bacteria as the index. The results showed that the optimal medium components were maltose 26.73 g/L， yeast extract 21.8 g/L， soybean peptone 16.59 g/L， MgSO4·7H2O 0.15 g/L， MnSO4·H2O 0.06 g/L， adenine 0.13 g/L， phenylalanine 0.051 g/L， and Tween 80 1 mL/L. The optimal culture conditions were as follows: initial pH 7.0， inoculum quantity 3%， culture temperature 30 ℃. The final viable bacteria count reached 9.2×109 CFU/mL， which laid a theoretical foundation for the high-density culture of Lactobacillus plantarium NCU137 and the preparation of high-activity bacteria.