虾夷扇贝裙边酶解物美拉德反应产物的自由基清除活性
作者:
作者单位:

(大连工业大学食品学院 国家海洋食品工程技术研究中心 辽宁大连 116034)

作者简介:

崔小凡(1996—),女,硕士生

通讯作者:

中图分类号:

基金项目:

国家重点研发计划项目(2018YFC0311205);辽宁省兴辽英才计划项目(XLYC1907101)


Free Radical Scavenging Activities of Maillard Reaction Products from Scallop (Patinopecten yessoensi) Mantle Hydrolysates
Author:
Affiliation:

(School of Food Science and Technology, National Engineering Research Center of Seafood,Dalian Polytechnic University, Dalian 116034, Liaoning)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    以虾夷扇贝裙边为原料,利用中性蛋白酶制备其酶解物及美拉德反应产物。扇贝裙边蛋白的条带主要分布在200,97 ku和42 ku。其经中性蛋白酶酶解3 h后,水解度达28.95%。将酶解物与核糖反应制备美拉德反应产物,并分析其抗氧化活性和风味物质组成。结果表明:美拉德反应生成较多的中间产物和褐色物质。经美拉德反应后,酶解物生成较多挥发性成分,尤其是杂环化合物种类增加。此外,酶解物的羟基和ABTS自由基清除能力仅分别为42.7%和31.64%,且未表现出明显的DPPH自由基清除能力。然而,经12 h的美拉德反应后,酶解物的羟自由基、DPPH和ABTS自由基清除能均力得到显著提高,表明扇贝裙边酶解物-核糖美拉德反应产物具有良好的抗氧化能力,可作为抗氧化剂应用于食品工业中。

    Abstract:

    In this paper, the scallop(Patinopecten yessoensi) mantle was used as the raw material, and the enzymatic hydrolysate was prepared by neutral protease. P. yessoensi mantle proteins (SMs) were primarily distributed 200, 97 ku and 42 ku. The SMs were hydrolyzed by neutrase for 3 h (SMHs) and the hydrolysis degree of SMHs reach 28.95%. Maillard reaction products(MRPs) were prepared by reacting ribose and SMHs, and its antioxidant activity and flavour composition were investigated. The Maillard reaction produced more intermediate products and brown matter, and the SMHs produced more volatile components after the Maillard reaction, especially an increased variety of heterocyclic compounds. Moreover, the SMHs had hydroxyl and ABTS radical scavenging capacity of 42.7% and 31.64%, respectively, but no DPPH scavenging ability. But after 12 hours of Maillard reaction, the hydroxyl, DPPH and ABTS radical scavenging capacity of the SMHs was significantly improved. These results suggested that the scallop mantle hydrolysates-ribose MRPs had potential antioxidant capacity and which had potential to use as an antioxidant in the food industry.

    参考文献
    相似文献
    引证文献
引用本文

崔小凡,杜椅楠,孙世广,韩佳润,阎佳楠,姜昕昱,李傲婷,吴海涛.虾夷扇贝裙边酶解物美拉德反应产物的自由基清除活性[J].中国食品学报,2022,22(8):276-284

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-08-07
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2022-09-08
  • 出版日期:
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知