This is a common phenomenon that multiple mycotoxins simultaneously exist in food which may heighten concerns about human or animal health owing to the combined toxicity of mycotoxins. Therefore， the detection of a single or the same type of mycotoxins can no longer fully meet the actual needs of ensuring food safety. There is an inevitable trend to develop a rapid and efficient method for simultaneous detection of multiple mycotoxins. This review sketched the characteristics of mycotoxin contamination， moreover， the research progress of sample pretreatment methods and detection techniques in simultaneous detection of multiple mycotoxins was also introduced. Liquid-liquid extraction， solid-phase extraction， immunoaffinity column and QuECHERS are widely used in the simultaneous extraction， enrichment， and purification of multiple mycotoxins. Ultra high performance liquid chromatography coupled with multi-level， high resolution mass spectrometry hold a unique advantage in the simultaneous detection of multiple mycotoxins and untargeted detection. This paper can provide reference for the research and application of simultaneous detection of multiple types of mycotoxins in food.