体外模拟大豆种皮多糖胃肠道消化特性研究
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(1.渤海大学食品科学与工程学院 辽宁锦州 121013 ;2.辽宁省粮谷类食品生物高效转化工程研究中心 辽宁锦州 121013;3.阜新和润生物技术有限公司 辽宁阜新 123000)

作者简介:

杨立娜(1987—),女,博士,副教授

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国家自然科学基金项目(31901680)


Studies on Gastrointestinal Digestion Characteristics of Soy Hull Polysaccharide by Simulation in Vitro
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(1.College of Food Science and Technology, Bohai University, Jinzhou 121013, Liaoning;2.Grain and Cereal Food Bio-efficient Transformation Engineering Research Center of Liaoning Province,Jinzhou 121013, Liaoning;3.Fuxin Herun Biotechnology Co., Ltd., Fuxin 123000, Liaoning)

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    摘要:

    为探究大豆种皮多糖在胃肠道的消化特征,采用体外模拟的方式进行分析,结果表明:大豆种皮多糖在模拟消化过程中,总糖和糖醛酸含量从口腔到小肠阶段无明显变化,其含量分别在126 mg和29 mg波动,而总糖含量在大肠阶段酵解12 h时开始下降,在酵解48 h后显著下降为(71.02±0.45) mg,还原糖含量由初始的(18.04±0.15) mg经历消化阶段后增加到(81.13±0.36) mg,在随后的酵解阶段最终降低为(51.55±1.31) mg;电位绝对值在消化阶段由3.46 mV增加到20.10 mV,在大肠阶段显著下降,酵解48 h后,电位绝对值变为15.26 mV;粒径在整个消化阶段呈明显的下降趋势,pH值在口腔到小肠阶段未发生显著变化,在大肠阶段由7.92下降到5.93;消化液黏度呈逐渐下降趋势。

    Abstract:

    In order to explore the digestive characteristics of soy hull polysaccharide in gastrointestinal tract, the results show that: the total sugar and uronic acid content of soy hull polysaccharide did not change significantly from oral to small intestine during simulated digestion, its content fluctuated between 126 mg and 29 mg, however, the total sugar content began to decrease at 12 h of fermentation in the large intestine stage and significantly decreased to (71.02 ± 0.45) mg after 48 h of fermentation, the content of reducing sugar increased from (18.04 ± 0.15) mg to (81.13 ± 0.36) mg after digestion, and finally decreased to (51.55 ± 1.31) mg in the subsequent fermentation stage; the absolute value of the potential increased from 3.46 mV to 20.10 mV in the digestion stage and decreased significantly in the large intestine stage. After 48 h of fermentation, the absolute value of the potential changed to 15.26 mV; the particle size showed an obvious downward trend during the whole digestion stage, and the pH value did not change significantly during the oral to small intestinal stages, and decreased from 7.92 to 5.93 in the large intestine stage; the viscosity of digestive juice showed a decreasing trend.

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杨立娜,王子义,蔡文琪,吴星会,王胜男,蔚彦平,周大宇,刘贺.体外模拟大豆种皮多糖胃肠道消化特性研究[J].中国食品学报,2022,22(9):55-62

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  • 收稿日期:2021-09-27
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  • 在线发布日期: 2022-10-26
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