In this study， quinoa was used as raw material， and the protein component with anti-colon cancer activity was screened by MTT method after further separation and purification by acetone-ammonium sulfate precipitation method through Q column. The anti-colon cancer activity of WQP was further investigated by cell model. The results of cell proliferation assay showed that WQP significantly inhibited the proliferation of colon cancer DLD1 and HCT-8 cells in a concentration gradient dependence， but had no effect on human normal colon epithelial cells FHC. Cell clone formation assay and cell morphology assay further proved that WQP had significant anti-colon cancer effect. Flow cytometry showed that WQP could induce apoptosis of colon cancer cells. In addition， cell scratch assay showed that WQP significantly restrained the migration of DLD1 and HCT-8 cells. Therefore， this study provides a theoretical basis for the development of WQP as a natural anti-colon cancer drug ingredient， and at the same time greatly improves the utilization rate of quinoa protein.