Objective: To investigate the preventive and protective effect of SCCL on acute alcoholic gastric ulcer in mice and its mechanism. Methods: The mice model of acute gastric ulcer was established by intragastric administration of 85% ethanol after fasting. The macroscopic state of the gastric tissue was evaluated and the ulcer index was calculated. The pathological changes of gastric mucosa cells were observed by H&E staining. The biochemical indexes related to oxidative stress and inflammatory factors were measured to evaluate the anti-oxidation and anti-inflammatory ability of sea cucumber cooking liquid on mice with acute alcoholic gastric ulcer. The expression of MAPK/NF-κB signaling pathway related genes in gastric tissues was determined by qRT-PCR. Results: Compared with model group， gastric ulcer index and gastric mucosal cell damage degree were significantly improved in SCCL group， oxidative stress factor and inflammatory factor in gastric tissue tended to normal levels， and the expression of MAPK/NF-κB related genes were down-regulated. Conclusion: SCCL can prevent and protect alcohol-induced acute gastric ulcer by improving oxidative stress and inflammation levels in mice.