This paper aims to explore the effect of aroniamelanocarpa juice on improving constipation and maintaining intestinal health in mice. The mice were divided into five groups: blank control group， constipation model control group and low， medium and high dose group of aroniamelanocarpa juice. After 14 days of sample intervention， the constipation model was established by loperamide hydrochloride. The defecation function index， intestinal motility index， gastrointestinal regulatory peptide index and 16S rRNA intestinal flora index of mice in each group were measured. The results showed that the constipation model was established. Aroniamelanocarpa juice could shorten the discharge time of the first black stool， increase the number and quality of defecation granules within 5 hours， improve the propulsion rate of small intestinal ink， promote the contents of gastrin (Gas)， motilin (MTL) and substance P (SP)， reduce the contents of somatostatin (SS)， endothelin (ET-1) and vasoactive intestinal peptide (VIP)， the effect has a certain dose correlation. In addition， aroniamelanocarpa juice can change the distribution of intestinal flora and enrich the abundance of species to a certain extent， significantly reduce the relative abundance ratio of Firmicutes and Bacteroidota(F/B)， reduce the abundance of Proteobacteria and Staphylococcus in constipated mice. Therefore， the study provides that aronia melanocarpa juice had a defecation effect on mice with constipation induced by loperamide hydrochloride， and its mechanism may be to improve the symptoms of constipation by regulating the secretion of intestinal hormones and the structure of intestinal flora.