In order to study the quality change of live Crassostrea gigas after post-harvesting， the simulated storage and transportation process of the transport chain of ‘waterless transport- re-immersed treatment - air exposure storage selling’ was set up. The shelf quality of live oyster was evaluated by three indexes including vitality， taste characteristic and microorganism. The experimental results showed that ① Vitality: after 1 day of waterless transportation， oyster weight， pH and SDH activity decreased significantly from 187.08 g， 6.78 and 7.92 mg/g to 183.39 g， 6.56 and 5.84 mg/g， respectively. After reimmersion by the artificial seawater， it quickly recovered to the initial state. Glycogen content and pH gradually decreased in the later air exposure storage process， but AEC value remained at about 40.00% all the time， and significantly decreased to 26.26% on the 10 th day of air exposure storage. ② Taste characteristics: taurine and glutamic acid contents were higher in the whole software， which were 2.76 mg/g and 1.05 mg/g， respectively， followed by aspartic acid and alanine， which were 0.43 mg/g and 0.49 mg/g， respectively. Only the TAV value of glutamate was >1， which was an important compound affecting the flavor of Crassostrea gigas. The content of glutamate in the simulated circulation was stable and did not fluctuate greatly. ③ Microorganism: The total number of bacteria increased significantly after 1 day of waterless transportation， but did not change significantly after re-immersion， and gradually increased in the later air exposure storage process. However， the total number of bacteria on the 10th day was 104/g， which was still within the edible 107/g range. High-throughput sequencing showed that the microbial community structure at the later stage of the simulated circulation process was dominated by Pseudoalteromonas and Shewanella， which may play an important role in the process of oyster spoilage. Therefore， the initial quality of Crassostrea gigas could be maintained under the mode of ‘1 d waterless transportation - re-immersed - air exposure storage selling 8 d’.