In the present work， the protective effect of corn glycopeptide intervention on alcohol-induced injury of LO₂ cells was studied， and the possible mechanism of corn glycopeptide antagonizing alcoholic liver cell injury was investigated. The results show that compared with the alcohol model group， corn glycopeptide at 50 μg/mL remarkably increased the activities of alcohol metabolism enzymes (ADH and ALDH) and antioxidant enzymes (SOD， CAT and GSH-Px) as well as GSH content， decreased ROS and MDA levels in damaged LO₂ cells. These results suggested that corn glycopeptide could enhance cytomembrane structural integrity by promoting alcohol metabolism and enhancing antioxidant activities， prevent the leakage of liver enzymes (AST， ALT and LDH) and reduce the percentage of early apoptosis and death of LO₂ cells to achieve the effect of protecting the LO₂ cells induced injury by alcohol.