In order to study the anti-inflammatory effect of Antarctic krill oil， the LPS-induced RAW264.7 cells were taken as the inflammatory model， the anti-inflammatory ability of Antarctic krill oil was evaluated by cell morphology， cell phagocytosis， myeloperoxidase(MPO) activity and pro-inflammatory cytokines (TNF-α， IL-1β and IL-6)， and its anti-inflammatory mechanism was preliminarily studied by the genes expression of pro-inflammatory cytokines and key inflammatory enzymes (iNOS and COX-2). The results showed that Antarctic krill oil could significantly alleviate the cell differentiation induced by LPS， inhibit the increase of cell phagocytosis， MPO activity and the secretion of inflammatory factors (especially TNF-α). The qRT-PCR detection indicated that Antarctic krill oil could significantly quell the activation of key inflammatory enzymes and inflammatory factor genes induced by LPS. This study shows that Antarctic krill oil has anti-inflammatory activity， which is related to its regulation of iNOS and COX-2.