Trichlorfon (TCF) is a highly effective and low-toxic pesticide. However， it can be converted into dichlorvos under alkaline environment， which increases toxicity. The detection of TCF is of great research importance. A flexible gold nanoplates (AuNTs)/tape based SERS substrates was fabricated. The target pesticide was extracted with adhesive tape from sample surface and then covered with AuNTs solution for SERS measurement. The AuNTs/tape SERS substrates was first assessed with good reproducibility and sensitivity using Nile Blue A (NBA) as Raman signal molecule. The intensity of TCF's Raman characteristic peak at 757 cm-1 showed a good linear correlation with the concentration of TCF solution ranging from 1 μg/mL to 10 μg/mL (y = 226.370x + 193.861， R2 = 0.9960). The LOD was calculated to be 0.781 μg/mL. The proposal of AuNTs/tape SERS substrates provide a novel method for pesticide detection.