Biological acid reduction is an important development direction of modern fruit wine green brewing. However， the current mechanism of some carboxylic acid transporters and their transport regulation is still incomplete， which severely limits the targeted breeding of high-efficiency acid-reducing strains. To this end， this experiment used a self-selected strain of Issatchenkia orientalis GS1-1 with good acid-reducing ability as the material， excavated its potential carboxylic acid transporter and identified it. First， the existing Issatchenkia orientalis genome sequence on NCBI was used to perform blast comparison with genes encoding different carboxylic acid transporters， and use bioinformatics techniques to perform gene comparison. Prediction of transmembrane helix， construction of phylogenetic tree， and use of green fluorescent protein for positioning. The haploid Saccharomyces cerevisiae BY4741 ADY2 and JEN1 carboxylic acid transporter double deletion engineering bacteria were constructed， and the plasmid containing the suspected target fragment was phenotype verified and specificity verified in Saccharomyces cerevisiae. The results showed that the target fragment 4 (OUT23260.1) was a malate carboxylic acid transporter； the target fragment 1 (XP_029319440.1) was a citrate transporter， and the functional region of the target fragment was located on the yeast cell membrane. This research has laid a theoretical foundation for exploring the acid reduction mechanism of non-Saccharomyces cerevisiae in the field of fermentation engineering.