In order to explore the effect of abscisic acid treatment on blueberry fruit during storage at low temperature stress， the Garden Blue blueberry were sprayed with 400， 600 μmol/L and 800 μmol/L abscisic acid solution， respectively. Firmness， total soluble solids content， membrane permeability， osmotic regulation substances， antioxidant substances content， antioxidant enzyme activity and other related physiological indexes were determined and principal component analysis and correlation analysis were carried out. The results showed that treated with 600 μmol/L abscisic acid then storage for 35 days， the firmness of blueberries decreased to 87.8% of the control group， and the soluble solids increased to 103.2% of the control group. The relative conductivity was 5.05% lower than that of the control group， and the content of MDA was 90.7% lower than that of the control group. The content of proline and soluble protein increased by 1.16 and 1.4 times respectively compared to the control group. The activities of SOD， CAT and APX were 1.51， 1.26 and 1.18 times higher than those of the control group， and the contents of anthocyanin and AsA were 1.72 and 1.34 times higher than those of the control group. Maintaining high antioxidant enzyme activity and antioxidant content improves the cold resistance of blueberry fruit and helps to maintain the quality of blueberry fruit. Principal component analysis and correlation analysis showed that relative conductivity (REC)， malondialdehyde (MDA)， proline(Pro)， and superoxide anion (O₂·-) were closely related to the cold resistance of blueberry fruit， and could be used as indicators to evaluate the cold resistance.