基于非靶向代谢组学分析黄桃果肉褐变过程中代谢产物的差异
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(1.湖南人文科技学院 农业与生物技术学院 湖南娄底 417000;2.广东省农业科学院果树研究所 农业农村部南亚热带果树生物学与遗传资源利用重点实验室广东省热带亚热带果树研究重点实验室 广州 510640)

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湖南省自然科学基金项目(2020JJ5270);湖南省教育厅科学研究项目(20A281);湖南省科技创新计划项目(2020RC1014);湖南省教育厅创新创业教育中心(湘教通〔2019〕333号);湖南省教育厅新农科研究与改革实践项目(湘教通〔2020〕94号);湖南创新型省份建设专项经费资助项目(2020NK4244);湖南省普通高等学校教学改革研究项目(HNJG-2020-0963)


Analysis of Metabolite Differences during the Browning Process of Yellow Peach Based on Untargeted Metabolomics
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(1.School of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology,Loudi 417000, Hunan;2.Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640)

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    摘要:

    以黄桃果实为试验材料,利用非靶向代谢组学研究其贮藏期间不同褐变程度果肉中的代谢物差异。采用主成分分析、聚类分析、正交偏最小二乘判别分析等方法分析不同褐变程度果肉中的差异代谢产物。结果表明:PE包装明显降低了黄桃果肉褐变指数,以无褐变的果肉为对照,共检出991种代谢物。在CK 12 d vs CK 0 d样本中,共检出206种显著上调代谢物和26种显著下调代谢物,而在PE 12 d vs CK 0 d样本中,共检出145种显著上调代谢物和58种显著下调代谢物。经HMDB通路分析,代谢物主要富集在13条通路中,其中,富集排名前6的通路分别是丙烷和聚酮化合物、脂质和类脂质分子、有机杂环化合物、有机酸及其衍生物、苯甲酸酯类、有机氧化合物。KEGG通路分析表明,代谢物主要富集在10条通路中,其中,富集排名前4的通路为其它次生代谢产物的生物合成、氨基酸代谢、辅因子和维生素的代谢通路,其它氨基酸代谢等通路。研究从代谢组学角度初步揭示了黄桃果肉褐变过程中代谢产物的差异性,为提高黄桃果实品质提供理论参考。

    Abstract:

    In this study, untargeted metabolomics method was used to identify and analysis the differences of metabolites during the browning of yellow peach. Yellow peach fruit were taken as experimental materialsl, and their metabolites were analyzed by principal component analysis (PCA), cluster analysis and orthogonal partial least squares discriminant analysis (OPLS-DA). The results indicated that pulp browning was significantly inhibited by PE packaging treatment. A total of 991 metabolites were detected in yellow peach fruit. 206 significantly up-regulated metabolites and 26 significantly down-regulated compounds were identified in the CK 12 d vs CK 0 d samples. While 145 significantly up-regulated metabolites and 58 significantly down-regulated compounds were identified in the PE 12 d vs CK 0 d samples. There were 13 HMDB metabolic pathways by using HMDB database. The first top 6 pathways were phenylpropanoids and polyketides, lipids and lipid-like molecules, organoheterocyclic, organic acids and derivatives, benzenoids and organic oxygen compounds. KEGG results showed that differential metabolites mainly distributed in 10 KEGG metabolic pathways, the first top 4 pathways were biosynthesis of other secondary metabolites, amino acid metabolism, metabolism of cofactors and vitamins and metabolism of other amino acids. This study revealed differential metabolites during the browning process of yellow peach, which could provide a theoretical reference for improving the quality of yellow peach fruit.

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龚意辉,李丽梅,黄华,李论,田宗琼,张娟,曾永贤,陈致印.基于非靶向代谢组学分析黄桃果肉褐变过程中代谢产物的差异[J].中国食品学报,2023,23(2):265-275

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  • 收稿日期:2022-02-02
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  • 在线发布日期: 2023-03-22
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