In this paper， the anti-tumor activities of different flavonoids from Youzao jujube were studied by cell proliferation， cell migration and cell apoptosis. All five flavonoids had inhibitory effect on cell growth of pancreatic cancer cell BXPC-3， colon cancer cell HT-29 and lung adenocarcinoma cell A549. Among the flavonoids， Quercetin had the most obvious inhibitory effect on BXPC-3 and HT-29， with inhibition rates 79.08% and 89.09%， respectively. Hyperoside had the most obvious inhibitory effect on A549， and the inhibitory rate was 74.32%. Quercetin had a strong inhibition on migration of BXPC-3， but no obvious effect on HT-29 and A549. TUNEL staining was used to access cell apoptosis. Results suggest that the inhibitory effect of of flavonoids of Youzao jujube may be mediated by cell apoptosis.