To explore the properties of active peptide in hairy tofu of fermented soybean curd，the process conditions， isolation and purification， antioxidant ability in vitro and amino acid composition of bioactive peptides in hairy tofu of fermented soybean curd were studied. The results showed that the highest content of bioactive peptide was from alkaline protease hydrolysis on the single enzyme method. The content of bioactive peptide from alkaline protease enzymolysis extraction was (69.441 ± 1.033) mg/g and 66.07% higher than that without alkaline protease， under the optimal extraction process conditions on response surface methodology as follows: material to liquid ratio 1∶50(g/mL)， enzyme amount 2 500 U/g， enzymolysis temperature 50.78 ℃， enzymatic hydrolysis pH 5.5， enzymolysis time 2.08 h. The total reducing force， the clearance of the ABTS， DPPH and hydroxyl radicals of the bioactive peptide from alkaline protease enzymolysis extraction were increased by 37.80%， 22.91%， 31.75% and 62.46% than that without alkaline protease， respectively. The bioactive peptide extraction with molecular <3 ku and 3-10 ku were sieved by ultrafiltration， than the same concentration enzyme lysates with out ultrafiltration， the clearance of the ABTS radicals increased by 145.26% and 74.71% and clearance of the DPPH radicals increased by 93.41% and 72.44% than the same concentration with out ultrafiltration. The amino acid composition of bioactive peptide extraction with alkaline protease under the optimal process were standard AA accounted to 73.01%， essential AA accounted to 37.74%， flavor AA accounted to 17.39% and officinal AA accounted to 43.67%. Luo's hairy toufu in Chengkan village of Huizhou district was an excellent source of food origin bioactive peptides with good antioxidant， nutritional and medicinal health functions， so it had great value in development and utilization.