蛋清蛋白Pickering乳液制备及其负载β-胡萝卜素的稳定性
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(武汉轻工大学食品科学与工程学院 武汉 430023)

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2018年度科技部创新方法工作专项(2018IM030100)


Preparation of Pickering Emulsion Stabilized by Egg White Protein Particles and the Stability of β-Carotene Loaded
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(School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023)

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    摘要:

    以蛋清蛋白凝胶颗粒为乳化剂制备Pickering乳液,研究油相比例、凝胶颗粒蛋白含量对蛋清蛋白颗粒稳定的Pickering乳液(EWP-PE)的影响,并探究其负载β-胡萝卜素的热稳定性及储藏稳定性的变化。结果表明:1)当蛋清蛋白含量4%,油相体积分数40%时,EWP-PE粒径47.37 μm,Zeta-电位值-30.3 mV,乳析指数14.2%,此时的EWP-PE具有较好的稳定性。2)EWP-PE负载β-胡萝卜素后,其粒径随温度的升高而显著增加(P<0.05)。在80℃条件下,乳液中β-胡萝卜素保留率达65.8%。储藏15 d后,乳液中β-胡萝卜素的保留率为42.8%,显著高于油相中β-胡萝卜素保留率(27.5%)。结论:负载β-胡萝卜素的EWP-PE具有较好的热稳定性和储藏稳定性。本研究结果为拓宽β-胡萝卜素在食品领域的应用提供了参考数据。

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    In this study, egg white protein gel particles were used as an emulsifier to prepare Pickering emulsion. The effects of oil phase ratio and particles concentration on egg white protein Pickering emulsion (EWP-PE) were studied. Loaded with β-carotene, the thermal stability and storage stability of the emulsion were studied. The results showed that: 1) When the content of egg white protein was 4% and the oil phase ratio was 40%, the particle size of EWP-PE was 47.37 μm, the Zeta- potential value was -30.3 mV and the emulsion index was 14.2%. At this condition, the EWP-PE had good stability. 2) When EWP-PE was loaded with β-carotene, its particle size increased significantly with temperature increasing (P<0.05). At 80 ℃, the retention rate of β-carotene in emulsion still maintained 65.8%. After 15 days of storage, the retention rate of β-carotene in EWP-PE was 42.8%, which was significantly higher than that in oil phase (27.5%). In conclusion, EWP-PE loaded with β-carotene showed good thermal stability and storage stability. This study provided reference data for the application of β-carotene in food field.

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潘晴楣,张志鹏,冉乐童,胥伟,王宏勋,易阳,郭丹郡.蛋清蛋白Pickering乳液制备及其负载β-胡萝卜素的稳定性[J].中国食品学报,2023,23(3):271-277

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  • 收稿日期:2022-03-23
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  • 在线发布日期: 2023-04-06
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