The dairy protein hydrolysis process can hydrolyze large molecule proteins into small molecule proteins and peptides. Infant formulas with partially hydrolyzed proteins may be beneficial to the digestion and absorption of infants， but the comparison between them and breast milk is unclear. In this study， a commercially available infant formula (EF) containing partially hydrolyzed protein was used to compare the in vitro digestion and absorption characteristics with standard infant formula (SF) and breast milk (BM)， and the amino acid score and digestible indispensable amino acid score were calculated based on the total amino acid pattern and digested amino acid pattern， respectively， to evaluate the protein quality. The results showed that the protein content of the two formulas was higher than that of BM， and the amino acid composition was close to that of BM. The in vitro protein digestibility was (33.50±0.39)%， (33.07±0.83)% and (38.49±0.51)% for EF， SF and BM， respectively， with the absorption rate of EF up to 78% of the BM. The results of digestible indispensable amino acid score showed that the limiting amino acids of EF were threonine and histidine， while all other amino acids scored above 75， which can be considered as good protein quality. Therefore， the infant formula containing partially hydrolyzed protein used in this study has better digestibility and absorption properties than the control formula and is closer to breast milk. This study can provide data to support the digestion and absorption characteristics of infant formula containing partially hydrolyzed protein and provide a theoretical basis for in vitro research methods for protein evaluation.