盐度对紫甘蓝酸菜发酵过程中挥发性风味物质及理化指标的影响
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(东北农业大学食品学院 哈尔滨 150030)

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Effect of Salinity on Volatile Flavor Compounds and Physicochemical Properties in Red Cabbage Sauerkraut during Fermentation
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(College of Food Science, Northeast Agricultural University, Harbin 150030)

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    摘要:

    为考察不同盐度(1%,3%,5%,10%)对紫甘蓝酸菜发酵过程中挥发性风味物质及理化指标的影响,利用顶空固相微萃取-气相色谱-质谱联用(HS-SPME/GC-MS)方法分析挥发性风味物质的差异性,比较在发酵过程中,其感官得分、pH、总酸、亚硝酸盐、氨基酸态氮、还原糖含量及乳酸菌数的差异。结果表明:盐度对紫甘蓝酸菜的感官得分及理化指标中的pH、总酸、氨基酸态氮、还原糖含量及乳酸菌数有显著影响(P<0.05)。盐度过高(>5%)不利于紫甘蓝酸菜的发酵及感官品质,其中3%盐度发酵酸菜的感官综合得分最高。HS-SPME/GC-MS分析结果表明,发酵末期(60 d),3%盐度发酵的酸菜的风味物质总含量最高;其中,部分烯烃类、醇类、醛类、酮类、异硫氰酸酯类及腈类等风味物质的含量显著高于其它盐度的酸菜(P<0.05),随着盐度的增加,这些风味物质相应减少。结合OAV值分析得到酸菜中21种关键挥发性风味物质,其在较高的盐度(>5%)下对应的OAV值显著降低(P<0.05);通过PCA可知,发酵中期(30 d)和末期(60 d),不同盐度发酵的酸菜间风味物质组成差异较大,其中3%盐度的风味物质组成更为丰富;Pearson相关性分析表明,盐度与总酸、氨基酸态氮含量、乳酸菌数、感官综合得分及大部分挥发性风味物质之间呈负相关,其中与总酸呈显著负相关(P<0.05),而与pH值、还原糖含量呈正相关。结论:相比其它盐度的酸菜,3%盐度发酵的紫甘蓝酸菜风味品质较好,可直接用于紫甘蓝的发酵工艺,对紫甘蓝酸菜的生产具有一定的指导意义。

    Abstract:

    In order to study the effects of different salinities on the volatile flavor compounds and physicochemical properties of red cabbage sauerkraut during the fermentation, there were four different salt concentrations of 1%, 3%, 5%, and 10% were designed. The volatile flavor compounds (VOCs) in red cabbage sauerkraut were detected by using the headspace solid-phase microextraction-gas chromatograph-mass spectrometry (HS-SPME/GC-MS) The differences of VOCs as well as sensory scores, pH, total acidity, nitrite, amino acid nitrogen, reducing sugar content and number of lactic acid bacteria (LAB) colonies in different samples were analyzed during the fermentation. The results showed that salinity had a significant effect on the sensory score of red cabbage sauerkraut and physicochemical indexes such as the pH, total acidity, amino acid nitrogen, reducing sugar content and the number of LAB colonies (P<0.05). High salinity (>5%) is not conducive to the fermentation and sensory quality of sauerkraut and fermented at 3% salinity has the highest sensory score. The results of HS-SPME/GC-MS analysis showed that at the end of fermentation (60 days), the total content of flavor substances in red cabbage sauerkraut fermented at 3% salinity was the highest. Among them, the content of some flavor compounds such as alkenes, alcohols, aldehydes, ketones, isothiocyanates and nitriles was significantly higher than that of sauerkraut with other salinity (P<0.05). With the increase of salinity, these flavor substances decreased accordingly. Combined with odour activity values (OAVs) analysis, 21 key VOCs in sauerkraut were obtained, and it was found that their OAVs would be significantly reduced (P<0.05) at higher salinity (>5%). According to principal component analysis (PCA), in the middle (30 days) and end (60 days) of fermentation, the component of flavor substances in sauerkraut fermented at different salinity was not quite similar, and the flavor substances at 3% salinity were more abundant. The Pearson correlation analysis disclosed that there was negative correlation between salinity and total acidity (P<0.05), amino acid nitrogen, LAB number, sensory scores as well as most volatile flavor compounds while the salinity was positively correlated to pH and reducing sugar content. Conclusion: Compared with other salinity, red cabbage fermented at 3% salinity has better flavor quality, and can be directly used in red cabbage sauerkraut making, which has certain guiding significance for processing red cabbage products.

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李智,解双瑜,孙波,柳凯,张宇.盐度对紫甘蓝酸菜发酵过程中挥发性风味物质及理化指标的影响[J].中国食品学报,2023,23(4):214-227

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  • 收稿日期:2022-04-12
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  • 在线发布日期: 2023-05-26
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