In order to study the changes of metabolites in winter jujube after cold storage， ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to analyze the metabolisms in postharvest winter jujube before and after cold storage. The metabolisms in cold stored differential times and without cold stored winter jujube were compared by principal component analysis and correlation analysis， orthogonal partial least squares discriminant analysis and other statistical analysis. The results showed that a total of 794 metabolites belonging to 13 kinds were identified in winter jujube， 93 and 154 differential metabolites were respectively screened out in refrigerated winter jujube for 60 d and 90 d. These results indicated that refrigeration had an impact on the quantity and content of differential metabolites， especially the content. The metabolic pathways of differential metabolites enrichment analysis found that the differential metabolites in the cold stored for 60 d and 90 d winter jujube were mainly in the metabolic pathway， the secondary metabolites biosynthesis pathway， linoleic acid metabolism pathway， flavonoid biosynthesis pathway， flavone and flavonol biosynthesis pathway. The results provided theoretical basis for studying the mechanism of fruit quality deterioration of cold-stored winter jujube.